Under electron microscope, growing chondrocytes, normal cartilage lacuna and developed organell which could work normally, were observed in the two repaired groups. 电镜观察表明,修复两组均有软骨细胞生成,有正常软骨陷窝,细胞器发达并能行使正常功能。
The result of electronic microscopic view after 20 weeks: Group C, the cartilage cell in cartilage lacuna, with advanced organelle and expanded? 20周透射电镜结果:C组软骨细胞位于软骨陷窝中,细胞器发达,粗面内质网成扩张状态。
Group B, the cartilage cell in cartilage lacuna, the organelle is less than group C. B组软骨细胞位于软骨陷窝中,细胞器较C组查差,可见线粒体空泡,有脂滴形成。
Histology examination The new cartilage in C group was bonded integratedly with the perimeter articular cartilage, a lot of hyaline chondrocyte and cartilage lacuna might be seen in these tissues. 组织学检查C组修复软骨与周围软骨融合较好,可见较多的透明软骨细胞及软骨陷窝;
The cartilage cells located inside the cartilage bone lacuna in the normal control group were in fusiform with complete cell membrane, even cytoplasm, less vacuoles in the cytoplasm and matrix. 正常对照组软骨细胞呈纺锤形,位于软骨陷窝内,胞膜完整,胞质均匀,胞质内少见空泡,基质少见。
At 12 weeks, the specimens in both positive control group and co-culture group formed cartilage-like tissue with mature cartilage lacuna and type ⅱ collagen expression in histology and immunohistochemistry. 体外培养12周后,阳性对照组(软骨细胞组)及共培养组均形成成熟的软骨样组织,组织学及免疫组化显示有成熟的软骨陷窝样结构及Ⅱ型胶原表达。
Results After 3 weeks, all cells were viable, formed cartilage lacuna, appeared homologous cell cluster, and secreted cartilage matrix stained by toluidine blue in culture. 结果体外培养3周,培养物内细胞均存活,形成软骨陷窝,同源性细胞簇出现,并分泌软骨基质。
The immunohistochemical staining of type II collagen: there was positive staining in all groups. The staining was heavy in the cytoplasm of cartilage cells and aroud the cartilage lacuna. II型胶原免疫组化染色:各组染色均为阳性,软骨细胞胞浆内及软骨陷窝周围深染。
Histologic observation shew that in group A, there were many osteoblast, a few cartilage cells, and bone lacuna in 4w.In 8w, osteocyte were mature, and bone trabecula can be seen. 组织学观察:A组4w可见大量成骨细胞,少量软骨细胞,形成骨陷窝结构。8w时可见骨细胞成熟,骨小梁结构出现,髓腔形成。